Speaker Biography

S.V. Chigrinets

Russia

Title: Effect of endocrine disrupting chemicals bisphenol A and triclosan on semen quality

S.V. Chigrinets
Biography:

To be updated

Abstract:

Background: The violation of the reproductive health of men is one of the urgent problems of medicine throughout the world. In this case, the most common form of male infertility is idiopathic one. The most likely causes of idiopathic infertility are considered oxidative stress, genetic factors, as well as endocrine disruptors. In this regard, it becomes relevant to study the effect of endocrine disruptors, in particular bisphenol A and triclosan, on male reproductive health.

Aims: To establish a relationship between the concentration of bisphenol A and triclosan in the seminal fluid and the quality of semen in men with normo- and pathozoospermia, as well as sperm DNA fragmentation.

Materials and methods: 84 samples of seminal fluid were studied in men with normo-and pathozoospermia. In the seminal fluid, the concentrations of bisphenol A and triclosan were determined by gas chromatography with mass spectrometry (GC-MS). For comparative analysis, patients were divided according to the degree of sperm DNA fragmentation into two groups: group 1 patients with sperm DNA fragmentation <15% (n = 18) and group 2 ≥ 15% (n = 29). Spermological research was carried out according to the recommendations of WHO (2010), taking into account the assessment of the number, motility and morphology of sperm, as well as the degree of fragmentation of sperm DNA. The results were considered statistically significant at p <0.05.

Results Bisphenol A and triclosan were found in 100% and 84.3% of ejaculate samples with a median concentration of 0.150 ng / ml and 0.11 ng / ml, respectively.

Using Spearman's rank correlation coefficient, statistically significant correlations were found between the concentration of bisphenol A and the concentration of spermatozoa (r = -0.309; p = 0.024), the proportion of progressively motile spermatozoa (r = -0.575; p <0.001), and the proportion of spermatozoa with normal morphology (r = -0.397; p = 0.003), as well as the degree of sperm DNA fragmentation (r = 0.349; p = 0.025). Triclosan was statistically significantly correlated with the proportion of sperm with normal morphology (r = -0.440; p = 0.004), as well as the degree of fragmentation of sperm DNA (r = 0.610; p <0.001).

Using the analysis of ROC curves to assess the risk of pathozoospermia in the ejaculate, the threshold concentration of bisphenol A and triclosan at the cut-off point was determined to be 0.103 ng / ml and 0.150 ng / ml, respectively. It was shown that the chances of developing increased DNA fragmentation in men with a concentration of triclosan in seminal fluid ≥0.11 ng / ml (Q2) are 18.3 times higher than among men with a lower concentration (OR = 18.3; 95% CI : 3.4-97.7) with differences being statistically significant (p <0.001). The concentration of BPA was statistically significantly higher in the group of men with a DNA fragmentation index ≥ 15% (p = 0.014), however, the chances of developing increased DNA fragmentation in men with a concentration of bisphenol in seminal fluid ≥0.15 ng / ml (Q2) were statistically insignificant.

Conclusions: Bisphenol A and triclosan in seminal fluid may negatively impact semen quality and damage sperm DNA.